Nanobiotechnology for health-care

C60 fullerene effect on the mechanokinetics of muscle gastrocnemius contraction in chronically alcoholized rats

O. Motuziuk1,2, D. Nozdrenko2, I. Vareniuk2, K. Bogutska2, Yu. Prylutskyy2

1Lesya Ukrainka Volyn National University, 43025 Lutsk, Ukraine.
E-mail: cmoplutsk@gmail.com
2Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine.
	
	The biomechanical parameters of muscle gastrocnemius contraction, biochemical indicators of blood and muscle tissue as well as histological changes in rat muscle tissue after chronic alcoholization for 3, 6 and 9 months were studied. The oral administration of C60 fullerene aqueous solution (C60FAS) at a dose of 1 mg/kg throughout the experiment was used as a therapeutic agent. C60FAS was shown to reduce the pathological processes development in the muscle apparatus by an average of 35-40% (Fig. 1). In particular, it reduced the time occurrence of fatigue processes in muscle during long-term development of alcoholic myopathy and inhibited oxidative processes in muscle, thereby preventing its degradation (Fig. 2). 
	Thus, these findings indicate promising prospects for the usage of water-soluble C60 fullerenes as potent antioxidants for the corrections of the pathological conditions of the muscle system arising from alcohol intoxication.
	Fig.1. The forces of the 1st (1*) and 10th (10*) contractions of the muscle gastrocnemius of alcoholised rats induced by 6 s non-relaxation stimulation pools at 50 Hz: alcoholization - rats treated with alcohol; alcoholization+C60 - rats treated with a mixture of alcohol and C60FAS at a dose of 1 mg/kg during the whole period of alcoholization; 3, 6 and 9 months - alcoholization lasting 3, 6 and 9 months, respectively.
	
		Fig.2. Representative histological images of muscle gastrocnemius: A and D – control group; B and E – alcoholization group; C and F – alcoholization+C60 group. A, B, C – hematoxylin and eosin staining; D, E, F – hematoxylin and picrofuchsin staining by van Gieson. Scale bar – 50 μm.