Nanobiotechnology for health-care

Peculiarities of silver nanoparticles synthesized using Artemisia vulgaris “hairy” root extracts
Duplij V.1, Kharchuk M.2, Kobylinska N.3, Matvieieva N.*1
1Institute of Cell Biology and Genetic Engineering NAS of Ukraine, 148 Zabolotny str, Kyiv-03143, Ukraine. E-mail: joyna@ukr.net
2D. K. Zabolotny Institute of Microbiology and Virology NAS of Ukraine, 152 Zabolotny str, Kyiv-03143, Ukraine.
3A.V.Dumansky Institute of Colloid Chemistry and Water Chemistry NAS of Ukraine, Ak. Vernadsky blvd. 42, Kyiv-03142, Ukraine

"Green" synthesis of metal nanoparticles (NPs) is becoming increasingly popular due to its environmental friendliness and low toxicity. One of the advantages of “green” synthesis is a high degree of size and shape control of the resulting NPs. Silver nanoparticles (AgNPs), the most widely studied type of NPs, can be synthesized in different sizes and shapes using a variety of plant extracts. These NPs tend to be more stable than those synthesized using chemical methods due to the complex combinations of compounds, such as polyphenols and flavonoids. The extracts from the “hairy” roots of Artemisia vulgaris L. were used to obtain and study AgNPs. TEM was applied to evaluate the size and shape of obtained AgNPs.The results demonstrated the differences in size and shape of NPs when the extracts of different “hairy” root lines (independent transformational events) were used. In particular, the use of flavonoid-rich “hairy” root water extracts allowed us to obtain smaller (2.5-49 nm, Fig a) NPs than using the extracts of mother plants (18-235 nm, Fig b). 

a

b

Figure - Size distribution of nanoparticles: NPs obtained using extract from Artemisia vulgaris "hairy" roots (a) and extract from control plants (b)
AgNPs synthesized using A. vulgaris “hairy” root extract due to a narrow range of nanoparticle sizes have a potential source of biocompatible, antimicrobial nanoparticles with potential applications in medicine and other fields.